/* * #%L * OME Bio-Formats package for reading and converting biological file formats. * %% * Copyright (C) 2005 - 2015 Open Microscopy Environment: * - Board of Regents of the University of Wisconsin-Madison * - Glencoe Software, Inc. * - University of Dundee * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 2 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * <http://www.gnu.org/licenses/gpl-2.0.html>. * #L% */ import java.util.Arrays; import loci.common.DateTools; import loci.common.services.ServiceFactory; import loci.formats.FormatReader; import loci.formats.IFormatReader; import loci.formats.ImageReader; import loci.formats.meta.IMetadata; import loci.formats.services.OMEXMLService; import ome.units.quantity.Length; import ome.units.quantity.Time; import ome.units.UNITS; /** * Uses Bio-Formats to extract some basic standardized * (format-independent) metadata. */ public class GetPhysicalMetadata { /** Outputs dimensional information. */ public static void printPixelDimensions(IFormatReader reader) { // output dimensional information int sizeX = reader.getSizeX(); int sizeY = reader.getSizeY(); int sizeZ = reader.getSizeZ(); int sizeC = reader.getSizeC(); int sizeT = reader.getSizeT(); int imageCount = reader.getImageCount(); System.out.println(); System.out.println("Pixel dimensions:"); System.out.println("\tWidth = " + sizeX); System.out.println("\tHeight = " + sizeY); System.out.println("\tFocal planes = " + sizeZ); System.out.println("\tChannels = " + sizeC); System.out.println("\tTimepoints = " + sizeT); System.out.println("\tTotal planes = " + imageCount); } /** Outputs global timing details. */ public static void printPhysicalDimensions(IMetadata meta, int series) { Length physicalSizeX = meta.getPixelsPhysicalSizeX(series); Length physicalSizeY = meta.getPixelsPhysicalSizeY(series); Length physicalSizeZ = meta.getPixelsPhysicalSizeZ(series); Time timeIncrement = meta.getPixelsTimeIncrement(series); System.out.println(); System.out.println("Physical dimensions:"); System.out.println("\tX spacing = " + physicalSizeX.value() + " " + physicalSizeX.unit().getSymbol()); System.out.println("\tY spacing = " + physicalSizeY.value() + " " + physicalSizeY.unit().getSymbol()); System.out.println("\tZ spacing = " + physicalSizeZ.value() + " " + physicalSizeZ.unit().getSymbol()); System.out.println("\tTime increment = " + timeIncrement.value(UNITS.S).doubleValue() + " seconds"); } public static void main(String[] args) throws Exception { // parse command line arguments if (args.length < 1) { System.err.println("Usage: java GetMetadata imageFile [seriesNo]"); System.exit(1); } String id = args[0]; int series = args.length > 1 ? Integer.parseInt(args[1]) : 0; // create OME-XML metadata store ServiceFactory factory = new ServiceFactory(); OMEXMLService service = factory.getInstance(OMEXMLService.class); IMetadata meta = service.createOMEXMLMetadata(); // create format reader IFormatReader reader = new ImageReader(); reader.setMetadataStore(meta); // initialize file System.out.println("Initializing " + id); reader.setId(id); int seriesCount = reader.getSeriesCount(); if (series < seriesCount) reader.setSeries(series); series = reader.getSeries(); System.out.println("\tImage series = " + series + " of " + seriesCount); printPixelDimensions(reader); printPhysicalDimensions(meta, series); } }