/* * #%L * OME Bio-Formats package for reading and converting biological file formats. * %% * Copyright (C) 2005 - 2015 Open Microscopy Environment: * - Board of Regents of the University of Wisconsin-Madison * - Glencoe Software, Inc. * - University of Dundee * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 2 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * <http://www.gnu.org/licenses/gpl-2.0.html>. * #L% */ package loci.formats.in; import java.io.IOException; import loci.common.RandomAccessInputStream; import loci.formats.CoreMetadata; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; /** * FEIReader is the file format reader for FEI and Philips .img files. */ public class FEIReader extends FormatReader { // -- Constants -- public static final String FEI_MAGIC_STRING = "XL"; private static final int INVALID_PIXELS = 112; // -- Fields -- private int headerSize; // -- Constructor -- /** Constructs a new FEI reader. */ public FEIReader() { super("FEI/Philips", "img"); suffixSufficient = false; domains = new String[] {FormatTools.SEM_DOMAIN}; } // -- IFormatReader API methods -- /* @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ @Override public boolean isThisType(RandomAccessInputStream stream) throws IOException { final int blockLen = 2; if (!FormatTools.validStream(stream, blockLen, false)) return false; return stream.readString(blockLen).startsWith(FEI_MAGIC_STRING); } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ @Override public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); in.seek(headerSize); byte[] segment = new byte[getSizeX() / 2]; byte[] plane = new byte[FormatTools.getPlaneSize(this)]; // interlace frames - there are four rows of two columns int halfRow = getSizeX() / 2; for (int q=0; q<4; q++) { for (int row=q; row<getSizeY(); row+=4) { for (int s=0; s<2; s++) { in.read(segment); in.skipBytes(INVALID_PIXELS / 2); for (int col=s; col<getSizeX(); col+=2) { plane[row*getSizeX() + col] = segment[col / 2]; } } } } RandomAccessInputStream pixels = new RandomAccessInputStream(plane); readPlane(pixels, x, y, w, h, buf); pixels.close(); return buf; } /* @see loci.formats.IFormatReader#close(boolean) */ @Override public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { headerSize = 0; } } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ @Override protected void initFile(String id) throws FormatException, IOException { super.initFile(id); in = new RandomAccessInputStream(id); in.order(true); CoreMetadata m = core.get(0); LOGGER.info("Reading file header"); if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) { in.skipBytes(44); float magnification = in.readFloat(); float kV = in.readFloat() / 1000; float wd = in.readFloat(); in.skipBytes(12); float spot = in.readFloat(); addGlobalMeta("Magnification", magnification); addGlobalMeta("kV", kV); addGlobalMeta("Working distance", wd); addGlobalMeta("Spot", spot); } in.seek(514); m.sizeX = in.readShort() - INVALID_PIXELS; m.sizeY = in.readShort(); in.skipBytes(4); headerSize = in.readShort(); // always one grayscale plane per file m.sizeZ = 1; m.sizeC = 1; m.sizeT = 1; m.imageCount = 1; m.littleEndian = true; m.pixelType = FormatTools.UINT8; m.rgb = false; m.indexed = false; m.interleaved = false; m.dimensionOrder = "XYCZT"; MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this); } }