/* * #%L * OME Bio-Formats package for reading and converting biological file formats. * %% * Copyright (C) 2005 - 2015 Open Microscopy Environment: * - Board of Regents of the University of Wisconsin-Madison * - Glencoe Software, Inc. * - University of Dundee * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 2 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * <http://www.gnu.org/licenses/gpl-2.0.html>. * #L% */ package loci.formats.in; import java.io.IOException; import java.util.ArrayList; import loci.common.Constants; import loci.common.RandomAccessInputStream; import loci.formats.CoreMetadata; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; /** * Zeiss LMS reader for data from Zeiss CSM 700 systems. Not to be confused * with the Zeiss LSM reader, which reads the much more common .lsm format */ public class ZeissLMSReader extends FormatReader { // -- Constants -- private static final String CHECK = "LMSFLE"; private static final String MARKER = "BM6"; // -- Fields -- private ArrayList<Long> offsets = new ArrayList<Long>(); private byte[][] lut; // -- Constructor -- /** Constructs a new LMS reader. */ public ZeissLMSReader() { super("Zeiss LMS", "lms"); domains = new String[] {FormatTools.LM_DOMAIN}; } // -- IFormatReader API methods -- /** @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ public boolean isThisType(RandomAccessInputStream stream) throws IOException { int checkLen = 16; if (!FormatTools.validStream(stream, checkLen, false)) { return false; } return in.readString(checkLen).indexOf(CHECK) >= 0; } /* @see loci.formats.IFormatReader#get8BitLookupTable() */ public byte[][] get8BitLookupTable() { FormatTools.assertId(currentId, true, 1); if (isIndexed()) { return lut; } return null; } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); in.seek(offsets.get(getSeriesCount() - getSeries() - 1)); in.skipBytes(no * FormatTools.getPlaneSize(this)); readPlane(in, x, y, w, h, buf); return buf; } /* @see loci.formats.IFormatReader#close(boolean) */ public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { offsets.clear(); lut = null; } } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ public void initFile(String id) throws FormatException, IOException { super.initFile(id); in = new RandomAccessInputStream(id); MetadataLevel level = getMetadataOptions().getMetadataLevel(); CoreMetadata m = core.get(0); CoreMetadata thumb = new CoreMetadata(); m.littleEndian = true; thumb.littleEndian = true; in.order(m.littleEndian); in.seek(18); double magnification = in.readInt(); // all assumed to be constant based upon the CSM 700 data sheet thumb.sizeX = 1280; thumb.sizeY = 1024; thumb.pixelType = FormatTools.UINT8; thumb.sizeC = 3; thumb.rgb = true; thumb.interleaved = true; thumb.dimensionOrder = "XYCZT"; m.sizeX = 1280; m.sizeY = 1024; m.pixelType = FormatTools.UINT16; m.sizeC = 1; m.rgb = false; m.dimensionOrder = "XYCZT"; m.indexed = true; // each image can be found using the "BM6" marker seekToNextMarker(); in.skipBytes(50); offsets.add(in.getFilePointer()); in.skipBytes(thumb.sizeX * thumb.sizeY * thumb.sizeC); seekToNextMarker(); in.skipBytes(50); lut = new byte[3][256]; for (int i=0; i<lut[0].length; i++) { for (int j=0; j<lut.length; j++) { lut[j][i] = in.readByte(); } in.skipBytes(1); // skip alpha channel } offsets.add(in.getFilePointer()); // again, Z stack is assumed based upon the CSM 700 data sheet thumb.sizeZ = 1; thumb.sizeT = 1; thumb.imageCount = thumb.sizeZ * thumb.sizeT; long availableBytes = in.length() - offsets.get(1); int planeSize = m.sizeX * m.sizeY * FormatTools.getBytesPerPixel(m.pixelType); m.sizeZ = (int) (availableBytes / planeSize); m.sizeT = 1; m.imageCount = m.sizeZ * m.sizeT; core.add(thumb); MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this); store.setInstrumentID(MetadataTools.createLSID("Instrument", 0), 0); String objective = MetadataTools.createLSID("Objective", 0, 0); store.setObjectiveID(objective, 0, 0); store.setObjectiveNominalMagnification(magnification, 0, 0); store.setObjectiveSettingsID(objective, 0); store.setObjectiveSettingsID(objective, 1); } private void seekToNextMarker() throws IOException { while (in.getFilePointer() < in.length()) { String check = in.readString(3); if (check.equals(MARKER)) { in.skipBytes(1); return; } in.seek(in.getFilePointer() - 2); } } }