/*
* #%L
* OME Bio-Formats package for reading and converting biological file formats.
* %%
* Copyright (C) 2005 - 2015 Open Microscopy Environment:
* - Board of Regents of the University of Wisconsin-Madison
* - Glencoe Software, Inc.
* - University of Dundee
* %%
* This program is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as
* published by the Free Software Foundation, either version 2 of the
* License, or (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public
* License along with this program. If not, see
* <http://www.gnu.org/licenses/gpl-2.0.html>.
* #L%
*/
package loci.formats.in;
import java.io.IOException;
import java.util.ArrayList;
import loci.common.Constants;
import loci.common.RandomAccessInputStream;
import loci.formats.CoreMetadata;
import loci.formats.FormatException;
import loci.formats.FormatReader;
import loci.formats.FormatTools;
import loci.formats.MetadataTools;
import loci.formats.meta.MetadataStore;
/**
* Zeiss LMS reader for data from Zeiss CSM 700 systems. Not to be confused
* with the Zeiss LSM reader, which reads the much more common .lsm format
*/
public class ZeissLMSReader extends FormatReader {
// -- Constants --
private static final String CHECK = "LMSFLE";
private static final String MARKER = "BM6";
// -- Fields --
private ArrayList<Long> offsets = new ArrayList<Long>();
private byte[][] lut;
// -- Constructor --
/** Constructs a new LMS reader. */
public ZeissLMSReader() {
super("Zeiss LMS", "lms");
domains = new String[] {FormatTools.LM_DOMAIN};
}
// -- IFormatReader API methods --
/** @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */
public boolean isThisType(RandomAccessInputStream stream) throws IOException {
int checkLen = 16;
if (!FormatTools.validStream(stream, checkLen, false)) {
return false;
}
return in.readString(checkLen).indexOf(CHECK) >= 0;
}
/* @see loci.formats.IFormatReader#get8BitLookupTable() */
public byte[][] get8BitLookupTable() {
FormatTools.assertId(currentId, true, 1);
if (isIndexed()) {
return lut;
}
return null;
}
/**
* @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int)
*/
public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h)
throws FormatException, IOException
{
FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h);
in.seek(offsets.get(getSeriesCount() - getSeries() - 1));
in.skipBytes(no * FormatTools.getPlaneSize(this));
readPlane(in, x, y, w, h, buf);
return buf;
}
/* @see loci.formats.IFormatReader#close(boolean) */
public void close(boolean fileOnly) throws IOException {
super.close(fileOnly);
if (!fileOnly) {
offsets.clear();
lut = null;
}
}
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
public void initFile(String id) throws FormatException, IOException {
super.initFile(id);
in = new RandomAccessInputStream(id);
MetadataLevel level = getMetadataOptions().getMetadataLevel();
CoreMetadata m = core.get(0);
CoreMetadata thumb = new CoreMetadata();
m.littleEndian = true;
thumb.littleEndian = true;
in.order(m.littleEndian);
in.seek(18);
double magnification = in.readInt();
// all assumed to be constant based upon the CSM 700 data sheet
thumb.sizeX = 1280;
thumb.sizeY = 1024;
thumb.pixelType = FormatTools.UINT8;
thumb.sizeC = 3;
thumb.rgb = true;
thumb.interleaved = true;
thumb.dimensionOrder = "XYCZT";
m.sizeX = 1280;
m.sizeY = 1024;
m.pixelType = FormatTools.UINT16;
m.sizeC = 1;
m.rgb = false;
m.dimensionOrder = "XYCZT";
m.indexed = true;
// each image can be found using the "BM6" marker
seekToNextMarker();
in.skipBytes(50);
offsets.add(in.getFilePointer());
in.skipBytes(thumb.sizeX * thumb.sizeY * thumb.sizeC);
seekToNextMarker();
in.skipBytes(50);
lut = new byte[3][256];
for (int i=0; i<lut[0].length; i++) {
for (int j=0; j<lut.length; j++) {
lut[j][i] = in.readByte();
}
in.skipBytes(1); // skip alpha channel
}
offsets.add(in.getFilePointer());
// again, Z stack is assumed based upon the CSM 700 data sheet
thumb.sizeZ = 1;
thumb.sizeT = 1;
thumb.imageCount = thumb.sizeZ * thumb.sizeT;
long availableBytes = in.length() - offsets.get(1);
int planeSize =
m.sizeX * m.sizeY * FormatTools.getBytesPerPixel(m.pixelType);
m.sizeZ = (int) (availableBytes / planeSize);
m.sizeT = 1;
m.imageCount = m.sizeZ * m.sizeT;
core.add(thumb);
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this);
store.setInstrumentID(MetadataTools.createLSID("Instrument", 0), 0);
String objective = MetadataTools.createLSID("Objective", 0, 0);
store.setObjectiveID(objective, 0, 0);
store.setObjectiveNominalMagnification(magnification, 0, 0);
store.setObjectiveSettingsID(objective, 0);
store.setObjectiveSettingsID(objective, 1);
}
private void seekToNextMarker() throws IOException {
while (in.getFilePointer() < in.length()) {
String check = in.readString(3);
if (check.equals(MARKER)) {
in.skipBytes(1);
return;
}
in.seek(in.getFilePointer() - 2);
}
}
}