/* * #%L * Bio-Formats Plugins for ImageJ: a collection of ImageJ plugins including the * Bio-Formats Importer, Bio-Formats Exporter, Bio-Formats Macro Extensions, * Data Browser and Stack Slicer. * %% * Copyright (C) 2006 - 2015 Open Microscopy Environment: * - Board of Regents of the University of Wisconsin-Madison * - Glencoe Software, Inc. * - University of Dundee * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 2 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * <http://www.gnu.org/licenses/gpl-2.0.html>. * #L% */ package loci.plugins.util; import ij.VirtualStack; import ij.process.ImageProcessor; import java.awt.image.IndexColorModel; import java.io.IOException; import java.util.ArrayList; import java.util.List; import loci.formats.ChannelMerger; import loci.formats.FormatException; import loci.formats.FormatTools; import loci.formats.IFormatReader; import loci.formats.Modulo; import loci.formats.cache.Cache; import loci.formats.cache.CacheException; import loci.formats.cache.CacheStrategy; import loci.formats.cache.CrosshairStrategy; import loci.plugins.util.RecordedImageProcessor.MethodEntry; /** * Subclass of VirtualStack that uses Bio-Formats to read planes on demand. * * @author Melissa Linkert melissa at glencoesoftware.com */ public class BFVirtualStack extends VirtualStack { // -- Fields -- protected ImageProcessorReader reader; protected String id; protected Cache cache; private List<List<MethodEntry>> methodStacks; private int currentSlice = -1; private RecordedImageProcessor currentProcessor; private boolean colorize; private boolean merge; private boolean record; private int series; private int[] len; private int[] planeIndexes; private float[] calibrationTable; // -- Static utility methods -- protected static int getWidth(IFormatReader r, String path, int series) throws FormatException, IOException { r.setSeries(series); return r.getSizeX(); } protected static int getHeight(IFormatReader r, String path, int series) throws FormatException, IOException { r.setSeries(series); return r.getSizeY(); } // -- Constructor -- public BFVirtualStack(String path, IFormatReader r, boolean colorize, boolean merge, boolean record) throws FormatException, IOException, CacheException { super(getWidth(r, path, r.getSeries()), getHeight(r, path, r.getSeries()), null, path); reader = new ImageProcessorReader(r); id = path; this.colorize = colorize; this.merge = merge && !r.isIndexed(); this.record = record; this.series = r.getSeries(); // set up cache int[] subC; Modulo moduloC = r.getModuloC(); if (moduloC.length() > 1) { subC = new int[] {r.getSizeC() / moduloC.length(), moduloC.length()}; } else { subC = new int[] {r.getSizeC()}; } if (merge) subC = new int[] {new ChannelMerger(r).getEffectiveSizeC()}; len = new int[subC.length + 2]; System.arraycopy(subC, 0, len, 0, subC.length); len[len.length - 2] = r.getSizeZ(); len[len.length - 1] = r.getSizeT(); CacheStrategy strategy = new CrosshairStrategy(len); cache = new Cache(strategy, new ImageProcessorSource(r), true); methodStacks = new ArrayList<List<MethodEntry>>(); for (int i=0; i<r.getImageCount(); i++) { methodStacks.add(new ArrayList<MethodEntry>()); } } // -- BFVirtualStack API methods -- public String getPath() { return id; } public ImageProcessorReader getReader() { return reader; } public Cache getCache() { return cache; } public RecordedImageProcessor getRecordedProcessor() { return currentProcessor; } public List<MethodEntry> getMethodStack() { if (currentSlice >= 0) return methodStacks.get(currentSlice); return null; } public void setPlaneIndexes(int[] planeIndexes) { this.planeIndexes = planeIndexes; } // -- VirtualStack API methods -- @Override public synchronized ImageProcessor getProcessor(int n) { reader.setSeries(series); // check cache first if (currentSlice >= 0 && currentProcessor != null) { List<MethodEntry> currentStack = currentProcessor.getMethodStack(); if (currentStack.size() > 1) { methodStacks.get(currentSlice).addAll(currentStack); } } int sliceIndex = planeIndexes == null ? n - 1 : planeIndexes[n - 1]; int[] pos = reader.getZCTCoords(sliceIndex); if (merge) pos = new ChannelMerger(reader).getZCTCoords(sliceIndex); int[] cachePos = FormatTools.rasterToPosition(len, sliceIndex); ImageProcessor ip = null; try { ip = (ImageProcessor) cache.getObject(cachePos); cache.setCurrentPos(cachePos); } catch (CacheException exc) { exc.printStackTrace(); } // cache missed try { if (ip == null) { ip = reader.openProcessors(reader.getIndex(pos[0], pos[1], pos[2]))[0]; } } catch (FormatException exc) { exc.printStackTrace(); } catch (IOException exc) { exc.printStackTrace(); } if (colorize) { // apply color table, if necessary byte[] lut = new byte[256]; byte[] blank = new byte[256]; for (int i=0; i<lut.length; i++) { lut[i] = (byte) i; blank[i] = (byte) 0; } IndexColorModel model = null; if (pos[1] < 3) { model = new IndexColorModel(8, 256, pos[1] == 0 ? lut : blank, pos[1] == 1 ? lut : blank, pos[1] == 2 ? lut : blank); } else model = new IndexColorModel(8, 256, lut, lut, lut); if (ip != null) ip.setColorModel(model); } else if (merge) { currentSlice = n - 1; ImageProcessor[] otherChannels = new ImageProcessor[reader.getSizeC() - 1]; for (int i=0; i<otherChannels.length; i++) { int channel = i >= pos[1] ? i + 1 : i; try { cachePos[0] = channel; otherChannels[i] = (ImageProcessor) cache.getObject(cachePos); } catch (CacheException exc) { exc.printStackTrace(); } if (otherChannels[i] == null) { try { int index = reader.getIndex(pos[0], channel, pos[2]); otherChannels[i] = reader.openProcessors(index)[0]; } catch (FormatException exc) { exc.printStackTrace(); } catch (IOException exc) { exc.printStackTrace(); } } } currentProcessor = new RecordedImageProcessor(ip, pos[1], otherChannels); currentProcessor.setDoRecording(record); if (calibrationTable == null) { calibrationTable = currentProcessor.getChild().getCalibrationTable(); } else { currentProcessor.setCalibrationTable(calibrationTable); } return currentProcessor.getChild(); } if (ip != null) { currentSlice = n - 1; currentProcessor = new RecordedImageProcessor(ip); currentProcessor.setDoRecording(record); if (calibrationTable == null) { calibrationTable = currentProcessor.getChild().getCalibrationTable(); } else { currentProcessor.setCalibrationTable(calibrationTable); } return currentProcessor.getChild(); } return null; } @Override public int getWidth() { reader.setSeries(series); return reader.getSizeX(); } @Override public int getHeight() { reader.setSeries(series); return reader.getSizeY(); } @Override public int getSize() { if (reader.getCurrentFile() == null) return 0; reader.setSeries(series); if (merge) return new ChannelMerger(reader).getImageCount(); return planeIndexes == null ? reader.getImageCount() : planeIndexes.length; } }