/* * #%L * OME Bio-Formats package for reading and converting biological file formats. * %% * Copyright (C) 2005 - 2015 Open Microscopy Environment: * - Board of Regents of the University of Wisconsin-Madison * - Glencoe Software, Inc. * - University of Dundee * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 2 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * <http://www.gnu.org/licenses/gpl-2.0.html>. * #L% */ package loci.formats.in; import java.io.IOException; import loci.common.RandomAccessInputStream; import loci.formats.CoreMetadata; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; import ome.units.quantity.ElectricPotential; import ome.units.quantity.Length; import ome.units.UNITS; import ome.xml.model.primitives.PositiveFloat; /** * AliconaReader is the file format reader for Alicona AL3D files. */ public class AliconaReader extends FormatReader { // -- Constants -- public static final String AL3D_MAGIC_STRING = "Alicona"; // -- Fields -- /** Image offset. */ private int textureOffset; /** Number of bytes per pixel (either 1 or 2). */ private int numBytes; // -- Constructor -- /** Constructs a new Alicona reader. */ public AliconaReader() { super("Alicona AL3D", "al3d"); domains = new String[] {FormatTools.SEM_DOMAIN}; } // -- IFormatReader API methods -- /* @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ @Override public boolean isThisType(RandomAccessInputStream stream) throws IOException { final int blockLen = 16; if (!FormatTools.validStream(stream, blockLen, false)) return false; return (stream.readString(blockLen)).indexOf(AL3D_MAGIC_STRING) >= 0; } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ @Override public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); int pad = (8 - (getSizeX() % 8)) % 8; int planeSize = (getSizeX() + pad) * getSizeY(); if (getPixelType() == FormatTools.FLOAT) { in.seek(textureOffset); readPlane(in, x, y, w, h, buf); return buf; } // 16-bit images are stored in a non-standard format: // all of the LSBs are stored together, followed by all of the MSBs // so instead of LMLMLM... storage, we have LLLLL...MMMMM... for (int i=0; i<numBytes; i++) { in.seek(textureOffset + (no * planeSize * (i + 1))); in.skipBytes(y * (getSizeX() + pad)); if (getSizeX() == w) { in.read(buf, i * w * h, w * h); } else { for (int row=0; row<h; row++) { in.skipBytes(x); in.read(buf, i * w * h + row * w, w); in.skipBytes(getSizeX() + pad - x - w); } } } if (numBytes > 1) { byte[] tmp = new byte[buf.length]; for (int i=0; i<planeSize; i++) { for (int j=0; j<numBytes; j++) { tmp[i*numBytes + j] = buf[planeSize*j + i]; } } System.arraycopy(tmp, 0, buf, 0, tmp.length); tmp = null; } return buf; } /* @see loci.formats.IFormatReader#close(boolean) */ @Override public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { textureOffset = numBytes = 0; } } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ @Override protected void initFile(String id) throws FormatException, IOException { super.initFile(id); in = new RandomAccessInputStream(id); CoreMetadata m = core.get(0); // check that this is a valid AL3D file LOGGER.info("Verifying Alicona format"); String magicString = in.readString(17); if (!magicString.trim().equals("AliconaImaging")) { throw new FormatException("Invalid magic string : " + "expected 'AliconaImaging', got " + magicString); } // now we read a series of tags // each one is 52 bytes - 20 byte key + 30 byte value + 2 byte CRLF LOGGER.info("Reading tags"); int count = 2; boolean hasC = false; String voltage = null, magnification = null, workingDistance = null; String pntX = null, pntY = null; int depthOffset = 0; for (int i=0; i<count; i++) { String key = in.readString(20).trim(); String value = in.readString(30).trim(); addGlobalMeta(key, value); in.skipBytes(2); if (key.equals("TagCount")) count += Integer.parseInt(value); else if (key.equals("Rows")) m.sizeY = Integer.parseInt(value); else if (key.equals("Cols")) m.sizeX = Integer.parseInt(value); else if (key.equals("NumberOfPlanes")) { m.imageCount = Integer.parseInt(value); } else if (key.equals("TextureImageOffset")) { textureOffset = Integer.parseInt(value); } else if (key.equals("TexturePtr") && !value.equals("7")) hasC = true; else if (key.equals("Voltage")) voltage = value; else if (key.equals("Magnification")) magnification = value; else if (key.equals("PixelSizeXMeter")) pntX = value; else if (key.equals("PixelSizeYMeter")) pntY = value; else if (key.equals("WorkingDistance")) workingDistance = value; else if (key.equals("DepthImageOffset")) { depthOffset = Integer.parseInt(value); } } LOGGER.info("Populating metadata"); if (textureOffset != 0) { numBytes = (int) (in.length() - textureOffset) / (getSizeX() * getSizeY() * getImageCount()); m.sizeC = hasC ? 3 : 1; m.sizeZ = 1; m.sizeT = getImageCount() / getSizeC(); m.pixelType = FormatTools.pixelTypeFromBytes(numBytes, false, false); } else { textureOffset = depthOffset; m.pixelType = FormatTools.FLOAT; m.sizeC = 1; m.sizeZ = 1; m.sizeT = 1; m.imageCount = 1; } m.rgb = false; m.interleaved = false; m.littleEndian = true; m.dimensionOrder = "XYCTZ"; m.metadataComplete = true; m.indexed = false; m.falseColor = false; MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this); // populate Image data if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) { // link Image and Instrument String instrumentID = MetadataTools.createLSID("Instrument", 0); store.setInstrumentID(instrumentID, 0); store.setImageInstrumentRef(instrumentID, 0); // populate Detector data // According to the spec, the voltage and magnification values are those // used when the dataset was acquired, i.e. detector settings. if (voltage != null) { store.setDetectorSettingsVoltage( new ElectricPotential(new Double(voltage), UNITS.V), 0, 0); // link DetectorSettings to an actual Detector String detectorID = MetadataTools.createLSID("Detector", 0, 0); store.setDetectorID(detectorID, 0, 0); store.setDetectorSettingsID(detectorID, 0, 0); // set required Detector type store.setDetectorType(getDetectorType("Other"), 0, 0); } // populate Objective data if (magnification != null) { store.setObjectiveCalibratedMagnification( new Double(magnification), 0, 0); } if (workingDistance != null) { store.setObjectiveWorkingDistance(new Length(new Double(workingDistance), UNITS.MICROM), 0, 0); } store.setObjectiveCorrection(getCorrection("Other"), 0, 0); store.setObjectiveImmersion(getImmersion("Other"), 0, 0); // link Objective to an Image using ObjectiveSettings String objectiveID = MetadataTools.createLSID("Objective", 0, 0); store.setObjectiveID(objectiveID, 0, 0); store.setObjectiveSettingsID(objectiveID, 0); // populate Dimensions data if (pntX != null && pntY != null) { double pixelSizeX = Double.parseDouble(pntX) * 1000000; double pixelSizeY = Double.parseDouble(pntY) * 1000000; Length sizeX = FormatTools.getPhysicalSizeX(pixelSizeX); Length sizeY = FormatTools.getPhysicalSizeY(pixelSizeY); if (sizeX != null) { store.setPixelsPhysicalSizeX(sizeX, 0); } if (sizeY != null) { store.setPixelsPhysicalSizeY(sizeY, 0); } } } } }