/* * #%L * OME Bio-Formats package for reading and converting biological file formats. * %% * Copyright (C) 2005 - 2015 Open Microscopy Environment: * - Board of Regents of the University of Wisconsin-Madison * - Glencoe Software, Inc. * - University of Dundee * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 2 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * <http://www.gnu.org/licenses/gpl-2.0.html>. * #L% */ package loci.formats.in; import java.io.File; import java.io.IOException; import java.util.ArrayList; import java.util.HashMap; import java.util.List; import loci.common.Constants; import loci.common.DataTools; import loci.common.DateTools; import loci.common.Location; import loci.common.RandomAccessInputStream; import loci.common.services.DependencyException; import loci.common.services.ServiceFactory; import loci.formats.CoreMetadata; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; import loci.formats.services.POIService; import loci.formats.tiff.IFD; import loci.formats.tiff.TiffParser; import ome.xml.model.enums.Binning; import ome.xml.model.primitives.PositiveFloat; import ome.xml.model.primitives.Timestamp; import ome.units.quantity.Length; import ome.units.quantity.Time; import ome.units.UNITS; /** * PCIReader is the file format reader for SimplePCI (Compix) .cxd files. * * @author Melissa Linkert melissa at glencoesoftware.com */ public class PCIReader extends FormatReader { // -- Constants -- public static final int PCI_MAGIC_BYTES = 0xd0cf11e0; // -- Fields -- private HashMap<Integer, String> imageFiles; private transient POIService poi; private HashMap<Integer, Double> timestamps; private String creationDate; private int binning; private List<Double> uniqueZ; // -- Constructor -- /** Constructs a new SimplePCI reader. */ public PCIReader() { super("Compix Simple-PCI", "cxd"); domains = new String[] {FormatTools.LM_DOMAIN}; } // -- IFormatReader API methods -- /* @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ @Override public boolean isThisType(RandomAccessInputStream stream) throws IOException { final int blockLen = 4; if (!FormatTools.validStream(stream, blockLen, false)) return false; return stream.readInt() == PCI_MAGIC_BYTES; } /* @see loci.formats.IFormatReader#getOptimalTileWidth() */ @Override public int getOptimalTileWidth() { FormatTools.assertId(currentId, true, 1); String file = imageFiles.get(0); try { if (poi == null) { initPOIService(); } RandomAccessInputStream s = poi.getDocumentStream(file); TiffParser tp = new TiffParser(s); if (tp.isValidHeader()) { IFD ifd = tp.getFirstIFD(); s.close(); return (int) ifd.getTileWidth(); } s.close(); } catch (FormatException e) { LOGGER.debug("Could not retrieve tile width", e); } catch (IOException e) { LOGGER.debug("Could not retrieve tile width", e); } return super.getOptimalTileWidth(); } /* @see loci.formats.IFormatReader#getOptimalTileHeight() */ @Override public int getOptimalTileHeight() { FormatTools.assertId(currentId, true, 1); String file = imageFiles.get(0); try { if (poi == null) { initPOIService(); } RandomAccessInputStream s = poi.getDocumentStream(file); TiffParser tp = new TiffParser(s); if (tp.isValidHeader()) { IFD ifd = tp.getFirstIFD(); s.close(); return (int) ifd.getTileLength(); } s.close(); } catch (FormatException e) { LOGGER.debug("Could not retrieve tile height", e); } catch (IOException e) { LOGGER.debug("Could not retrieve tile height", e); } return super.getOptimalTileHeight(); } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ @Override public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); if (poi == null) { initPOIService(); } String file = imageFiles.get(no); RandomAccessInputStream s = poi.getDocumentStream(file); TiffParser tp = new TiffParser(s); // can be raw pixel data or an embedded TIFF file if (tp.isValidHeader()) { IFD ifd = tp.getFirstIFD(); tp.getSamples(ifd, buf, x, y, w, h); } else { s.seek(0); readPlane(s, x, y, w, h, buf); } s.close(); return buf; } /* @see loci.formats.IFormatReader#close(boolean) */ @Override public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { imageFiles = null; timestamps = null; if (poi != null) poi.close(); poi = null; binning = 0; creationDate = null; uniqueZ = null; } } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ @Override protected void initFile(String id) throws FormatException, IOException { super.initFile(id); imageFiles = new HashMap<Integer, String>(); timestamps = new HashMap<Integer, Double>(); uniqueZ = new ArrayList<Double>(); CoreMetadata m = core.get(0); initPOIService(); double scaleFactor = 1; final List<String> allFiles = poi.getDocumentList(); if (allFiles.isEmpty()) { throw new FormatException( "No files were found - the .cxd may be corrupt."); } double firstZ = 0d, secondZ = 0d; int mode = 0; for (String name : allFiles) { int separator = name.lastIndexOf(File.separator); String parent = name.substring(0, separator); String relativePath = name.substring(separator + 1); RandomAccessInputStream stream = null; if (!(relativePath.startsWith("Bitmap") || (relativePath.equals("Data") && parent.indexOf("Image") != -1))) { stream = poi.getDocumentStream(name); stream.order(true); } if (stream != null && stream.length() == 8) { double value = stream.readDouble(); stream.seek(0); String key = name.replace(File.separatorChar, ' '); key = key.replaceAll("Root Entry ", ""); key = key.replaceAll("Field Data ", ""); key = key.replaceAll("Details ", ""); addGlobalMeta(key, value); } if (relativePath.equals("Field Count")) { m.imageCount = stream.readInt(); } else if (relativePath.equals("File Has Image")) { if (stream.readShort() == 0) { throw new FormatException("This file does not contain image data."); } } else if (relativePath.startsWith("Bitmap") || (relativePath.equals("Data") && parent.indexOf("Image") != -1)) { imageFiles.put(imageFiles.size(), name); if (getSizeX() != 0 && getSizeY() != 0) { int bpp = FormatTools.getBytesPerPixel(getPixelType()); int plane = getSizeX() * getSizeY() * bpp; if (getSizeC() == 0 || getSizeC() * plane > poi.getFileSize(name)) { m.sizeC = poi.getFileSize(name) / plane; } } } else if (relativePath.indexOf("Image_Depth") != -1) { boolean firstBits = m.bitsPerPixel == 0; int bits = (int) stream.readDouble(); m.bitsPerPixel = bits; while (bits % 8 != 0 || bits == 0) bits++; if (bits % 3 == 0) { m.sizeC = 3; bits /= 3; m.bitsPerPixel /= 3; } bits /= 8; m.pixelType = FormatTools.pixelTypeFromBytes(bits, false, false); if (getSizeC() > 1 && firstBits) { m.sizeC /= bits; } } else if (relativePath.indexOf("Image_Height") != -1 && getSizeY() == 0) { m.sizeY = (int) stream.readDouble(); } else if (relativePath.indexOf("Image_Width") != -1 && getSizeX() == 0) { m.sizeX = (int) stream.readDouble(); } else if (relativePath.indexOf("Time_From_Start") != -1) { timestamps.put(getTimestampIndex(parent), stream.readDouble()); } else if (relativePath.indexOf("Position_Z") != -1) { double zPos = stream.readDouble(); if (!uniqueZ.contains(zPos) && getSizeZ() <= 1) { uniqueZ.add(zPos); } if (name.indexOf("Field 1" + File.separator) != -1) firstZ = zPos; else if (name.indexOf("Field 2" + File.separator) != -1) secondZ = zPos; } else if (relativePath.equals("First Field Date & Time")) { long date = (long) stream.readDouble() * 1000; creationDate = DateTools.convertDate(date, DateTools.COBOL); } else if (relativePath.equals("GroupMode")) { mode = stream.readInt(); } else if (relativePath.equals("GroupSelectedFields")) { m.sizeZ = (int) (stream.length() / 8); } else if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) { if (relativePath.equals("Binning")) { binning = (int) stream.readDouble(); } else if (relativePath.equals("Comments")) { String comments = stream.readString((int) stream.length()); String[] lines = comments.split("\n"); for (String line : lines) { int eq = line.indexOf("="); if (eq != -1) { String key = line.substring(0, eq).trim(); String value = line.substring(eq + 1).trim(); addGlobalMeta(key, value); if (key.equals("factor")) { if (value.indexOf(";") != -1) { value = value.substring(0, value.indexOf(";")); } scaleFactor = Double.parseDouble(value.trim()); } } } } } if (stream != null) { stream.close(); } } boolean zFirst = Math.abs(firstZ - secondZ) > Constants.EPSILON; if (getSizeC() == 0) m.sizeC = 1; if (mode == 0) { m.sizeZ = 0; } if (getSizeZ() <= 1 || (getImageCount() % getSizeZ()) != 0) { m.sizeZ = uniqueZ.isEmpty() ? 1 : uniqueZ.size(); } m.sizeT = getImageCount() / getSizeZ(); while (getSizeZ() * getSizeT() < getImageCount()) { m.sizeZ++; m.sizeT = getImageCount() / getSizeZ(); } m.rgb = getSizeC() > 1; if (imageFiles.size() > getImageCount() && getSizeC() == 1) { m.sizeC = imageFiles.size() / getImageCount(); m.imageCount *= getSizeC(); } else { m.imageCount = getSizeZ() * getSizeT(); } m.interleaved = false; m.dimensionOrder = zFirst ? "XYCZT" : "XYCTZ"; m.littleEndian = true; m.indexed = false; m.falseColor = false; m.metadataComplete = true; // re-index image files String[] files = imageFiles.values().toArray(new String[imageFiles.size()]); for (String file : files) { int separator = file.lastIndexOf(File.separator); String parent = file.substring(0, separator); imageFiles.put(getImageIndex(parent), file); } int bpp = FormatTools.getBytesPerPixel(m.pixelType); int expectedPlaneSize = m.sizeX * m.sizeY * bpp * m.sizeC; String file = imageFiles.get(0); RandomAccessInputStream s = poi.getDocumentStream(file); TiffParser tp = new TiffParser(s); // don't correct the image width if it's stored as a TIFF if (!tp.isValidHeader() && s.length() > expectedPlaneSize) { m.sizeX += (s.length() - expectedPlaneSize) / (m.sizeY * bpp * m.sizeC); } s.close(); MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this, true); if (creationDate != null) { store.setImageAcquisitionDate(new Timestamp(creationDate), 0); } if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) { Length sizeX = FormatTools.getPhysicalSizeX(scaleFactor); Length sizeY = FormatTools.getPhysicalSizeY(scaleFactor); if (sizeX != null) { store.setPixelsPhysicalSizeX(sizeX, 0); } if (sizeY != null) { store.setPixelsPhysicalSizeY(sizeY, 0); } for (int i=0; i<timestamps.size(); i++) { if (i >= getImageCount()) { break; } Double timestamp = timestamps.get(i); if (timestamp != null) { store.setPlaneDeltaT(new Time(timestamp, UNITS.S), 0, i); } if (i == 2) { Double first = timestamps.get(1); Double increment = timestamp - first; if (increment != null) { store.setPixelsTimeIncrement(new Time(increment, UNITS.S), 0); } } } if (binning > 0) { String instrumentID = MetadataTools.createLSID("Instrument", 0); String detectorID = MetadataTools.createLSID("Detector", 0); store.setInstrumentID(instrumentID, 0); store.setDetectorID(detectorID, 0, 0); store.setDetectorType(getDetectorType("Other"), 0, 0); store.setImageInstrumentRef(instrumentID, 0); Binning binningEnum = getBinning(binning + "x" + binning); for (int c=0; c<getEffectiveSizeC(); c++) { store.setDetectorSettingsID(detectorID, 0, c); store.setDetectorSettingsBinning(binningEnum, 0, c); } } } } // -- Helper methods -- private void initPOIService() throws FormatException, IOException { try { ServiceFactory factory = new ServiceFactory(); poi = factory.getInstance(POIService.class); } catch (DependencyException de) { throw new FormatException("POI library not found", de); } poi.initialize(Location.getMappedId(getCurrentFile())); } /** Get the image index from the image file name. */ private Integer getImageIndex(String path) { int space = path.lastIndexOf(" ") + 1; if (space >= path.length()) return null; int end = path.indexOf(File.separator, space); String field = path.substring(space, end); String image = "1"; int imageIndex = path.indexOf("Image") + 5; if (imageIndex >= 0) { end = path.indexOf(File.separator, imageIndex); if (end < 0) end = path.length(); image = path.substring(imageIndex, end); } try { int channel = Integer.parseInt(image) - 1; return getEffectiveSizeC() * (Integer.parseInt(field) - 1) + channel; } catch (NumberFormatException e) { } return null; } private Integer getTimestampIndex(String path) { int space = path.lastIndexOf(" ") + 1; if (space >= path.length()) return null; int end = path.indexOf(File.separator, space); return Integer.parseInt(path.substring(space , end)) - 1; } }