/* * #%L * OME Bio-Formats package for reading and converting biological file formats. * %% * Copyright (C) 2005 - 2015 Open Microscopy Environment: * - Board of Regents of the University of Wisconsin-Madison * - Glencoe Software, Inc. * - University of Dundee * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 2 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * <http://www.gnu.org/licenses/gpl-2.0.html>. * #L% */ package loci.formats.in; import java.io.IOException; import ome.xml.model.primitives.Timestamp; import loci.common.DateTools; import loci.common.Location; import loci.common.RandomAccessInputStream; import loci.formats.CoreMetadata; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; import ome.xml.model.primitives.PositiveFloat; import ome.units.quantity.Length; import ome.units.UNITS; /** * GatanDM2Reader is the file format reader for Gatan .dm2 files. */ public class GatanDM2Reader extends FormatReader { // -- Constants -- private static final int HEADER_SIZE = 24; private static final int DM2_MAGIC_BYTES = 0x3d0000; // -- Fields -- private Double pixelSizeX; private Double pixelSizeY; private String pixelSizeUnits; // -- Constructor -- /** Constructs a new Gatan .dm2 reader. */ public GatanDM2Reader() { super("Gatan DM2", "dm2"); domains = new String[] {FormatTools.SPM_DOMAIN}; suffixNecessary = false; } // -- IFormatReader API methods -- /* @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ @Override public boolean isThisType(RandomAccessInputStream stream) throws IOException { final int blockLen = 4; if (!FormatTools.validStream(stream, blockLen, false)) return false; return stream.readInt() == DM2_MAGIC_BYTES; } /* @see loci.formats.IFormatReader#close(boolean) */ @Override public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { pixelSizeX = null; pixelSizeY = null; pixelSizeUnits = null; } } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ @Override public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); in.seek(HEADER_SIZE); readPlane(in, x, y, w, h, buf); return buf; } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ @Override protected void initFile(String id) throws FormatException, IOException { super.initFile(id); in = new RandomAccessInputStream(id); in.setEncoding("ISO-8859-1"); int magicBytes = in.readInt(); if (magicBytes != DM2_MAGIC_BYTES) { throw new FormatException("Invalid DM2 file"); } in.skipBytes(8); long footerOffset = in.readInt() + 16; CoreMetadata m = core.get(0); m.sizeX = in.readShort(); m.sizeY = in.readShort(); int bpp = in.readShort(); boolean signed = in.readShort() == 1; m.pixelType = FormatTools.pixelTypeFromBytes(bpp, signed, true); m.sizeC = 1; m.sizeT = 1; m.sizeZ = 1; m.imageCount = 1; m.dimensionOrder = "XYZCT"; m.littleEndian = false; MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this); String instrumentID = MetadataTools.createLSID("Instrument", 0); store.setInstrumentID(instrumentID, 0); store.setImageInstrumentRef(instrumentID, 0); String date = null, time = null, name = null; in.skipBytes(FormatTools.getPlaneSize(this) + 35); MetadataLevel level = getMetadataOptions().getMetadataLevel(); while (in.getFilePointer() < in.length()) { if (level == MetadataLevel.MINIMUM && date != null && time != null && name != null) { break; } int strlen = in.readShort(); if (strlen == 0 || strlen > 255) { in.skipBytes(35); strlen = in.readShort(); if (strlen < 0 || strlen + in.getFilePointer() >= in.length()) { in.seek(in.getFilePointer() - 10); strlen = in.readShort(); } } if (strlen < 0 || strlen + in.getFilePointer() >= in.length()) break; String label = in.readString(strlen); StringBuffer value = new StringBuffer(); int block = in.readInt(); if (block == 5) { in.skipBytes(33); if (in.readShort() == 0) { if (in.readShort() == 39) { in.skipBytes(1); } else { in.skipBytes(2); } } else { in.seek(in.getFilePointer() - 2); continue; } } else if (block == 0 || (block > 0xffff && block < 0x01000000)) { if (block != 0 && strlen > 0) { in.seek(in.getFilePointer() - 4); value.append(label); label = "Description"; addGlobalMeta(label, value.toString()); } else if (block != 0) { in.skipBytes(15); } parseExtraTags(); continue; } else if (block >= 0x01000000) { in.skipBytes(34); strlen = in.readShort(); if (strlen + in.getFilePointer() >= in.length()) { break; } label = in.readString(strlen); block = in.readInt(); if (block == 5) { in.skipBytes(33); continue; } } int len = in.readInt(); if (len + in.getFilePointer() >= in.length() || len < 0) break; String type = in.readString(len); int extra = in.readInt() - 2; int count = in.readInt(); if (type.equals("TEXT")) { value.append(in.readString(count)); if (block == 5) { in.skipBytes(22); if (in.readInt() == 4) { if (in.readString(4).equals("TEXT")) { in.skipBytes(4); count = in.readInt(); value.append(", " + in.readString(count)); in.skipBytes(37); } else { in.skipBytes(7); } } else { in.skipBytes(11); } } } else if (type.equals("long")) { count /= 8; for (int i=0; i<count; i++) { value.append(in.readLong()); if (i < count - 1) value.append(", "); } in.skipBytes(4); } else if (type.equals("bool")) { for (int i=0; i<count; i++) { value.append(in.read() == 1); if (i < count - 1) value.append(", "); } } else if (type.equals("shor")) { count /= 2; for (int i=0; i<count; i++) { value.append(in.readShort()); if (i < count - 1) value.append(", "); } } else if (type.equals("sing")) { count /= 4; for (int i=0; i<count; i++) { value.append(in.readFloat()); if (i < count - 1) value.append(", "); } } else { if (count < 0 || count + in.getFilePointer() > in.length()) { break; } in.skipBytes(count); } in.skipBytes(16); addGlobalMeta(label, value.toString()); if (label.equals("Acquisition Date")) { date = value.toString(); if (date != null && date.indexOf("/") != -1) { // if the year is stored as a single digit, then it will be parsed // literally, e.g. '7' -> '0007', when we want '7' -> '2007' String year = date.substring(date.lastIndexOf("/") + 1); if (year.length() < 2) { year = "0" + year; } date = date.substring(0, date.lastIndexOf("/") + 1) + year; } } else if (label.equals("Acquisition Time")) { time = value.toString(); } else if (label.equals("Binning")) { int bin = (int) Double.parseDouble(value.toString()); store.setDetectorSettingsBinning(getBinning(bin + "x" + bin), 0, 0); String detectorID = MetadataTools.createLSID("Detector", 0, 0); store.setDetectorID(detectorID, 0, 0); store.setDetectorSettingsID(detectorID, 0, 0); } else if (label.equals("Name")) { name = value.toString(); } else if (label.equals("Operator")) { String[] experimenterName = value.toString().split(" "); store.setExperimenterFirstName(experimenterName[0], 0); if (experimenterName.length > 1) { store.setExperimenterLastName(experimenterName[1], 0); } String expID = MetadataTools.createLSID("Experimenter", 0); store.setExperimenterID(expID, 0); store.setImageExperimenterRef(expID, 0); } } if (date != null && time != null) { String[] format = new String[] { "M/d/yy h:mm:ss a", "d/M/yy h:mm:ss a", "M/d/yy H:mm:ss", "d/M/yy H:mm:ss"}; date += " " + time; date = DateTools.formatDate(date, format); if (date != null) { store.setImageAcquisitionDate(new Timestamp(date), 0); } } if (name != null) { store.setImageName(name, 0); } if (pixelSizeX != null) { store.setPixelsPhysicalSizeX(FormatTools.createLength(pixelSizeX, UNITS.MICROM), 0); } if (pixelSizeY != null) { store.setPixelsPhysicalSizeY(FormatTools.createLength(pixelSizeY, UNITS.MICROM), 0); } } // -- Helper methods -- private void parseExtraTags() throws IOException { while (in.getFilePointer() < in.length()) { int tag = in.readShort(); int length = in.readInt(); String value = "foo"; if (length == 4) { value = String.valueOf(in.readFloat()); } else if (length == 2) { value = String.valueOf(in.readShort()); } else if (length == 1) { value = String.valueOf(in.read()); } else { value = in.readString(length); int nullIndex = value.indexOf("\0"); if (nullIndex >= 0) { value = value.substring(0, nullIndex); } } value = value.trim(); String label = "Tag " + Integer.toHexString(tag); switch (tag) { case 17: label = "BlackContrastLimit"; break; case 18: label = "WhiteContrastLimit"; break; case 22: label = "Scale"; break; case 27: label = "MaxPixelValue"; break; case 28: label = "MinPixelValue"; break; case 31: label = "Physical width"; pixelSizeX = new Double(value); break; case 32: label = "Physical height"; pixelSizeY = new Double(value); break; case 37: label = "Image label"; break; case 38: label = "MinimumContrast"; break; case 53: label = "Physical size units"; pixelSizeUnits = value; break; case 62: label = "Origin"; break; } addGlobalMeta(label, value); } if (pixelSizeUnits != null && !pixelSizeUnits.equals("µm")) { LOGGER.warn("Unsupported units: " + pixelSizeUnits); } } }