// // ChannelSeparator.java // /* OME Bio-Formats package for reading and converting biological file formats. Copyright (C) 2005-@year@ UW-Madison LOCI and Glencoe Software, Inc. This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA */ package loci.formats; import java.io.IOException; /** * Logic to automatically separate the channels in a file. * * <dl><dt><b>Source code:</b></dt> * <dd><a href="http://trac.openmicroscopy.org.uk/ome/browser/bioformats.git/components/bio-formats/src/loci/formats/ChannelSeparator.java">Trac</a>, * <a href="http://git.openmicroscopy.org/?p=bioformats.git;a=blob;f=components/bio-formats/src/loci/formats/ChannelSeparator.java;hb=HEAD">Gitweb</a></dd></dl> */ public class ChannelSeparator extends ReaderWrapper { // -- Utility methods -- /** Converts the given reader into a ChannelSeparator, wrapping if needed. */ public static ChannelSeparator makeChannelSeparator(IFormatReader r) { if (r instanceof ChannelSeparator) return (ChannelSeparator) r; return new ChannelSeparator(r); } // -- Fields -- /** Last image opened. */ private byte[] lastImage; /** Index of last image opened. */ private int lastImageIndex = -1; /** Series of last image opened. */ private int lastImageSeries = -1; /** X index of last image opened. */ private int lastImageX = -1; /** Y index of last image opened. */ private int lastImageY = -1; /** Width of last image opened. */ private int lastImageWidth = -1; /** Height of last image opened. */ private int lastImageHeight = -1; // -- Constructors -- /** Constructs a ChannelSeparator around a new image reader. */ public ChannelSeparator() { super(); } /** Constructs a ChannelSeparator with the given reader. */ public ChannelSeparator(IFormatReader r) { super(r); } // -- ChannelSeparator API methods -- /** * Returns the image number in the original dataset that corresponds to the * given image number. For instance, if the original dataset was a single * RGB image and the given image number is 2, the return value will be 0. * * @param no is an image number greater than or equal to 0 and less than * getImageCount() * @return the corresponding image number in the original (unseparated) data. */ public int getOriginalIndex(int no) { int imageCount = getImageCount(); int originalCount = reader.getImageCount(); if (imageCount == originalCount) return no; int[] coords = getZCTCoords(no); coords[1] /= reader.getRGBChannelCount(); return reader.getIndex(coords[0], coords[1], coords[2]); } // -- IFormatReader API methods -- /* @see IFormatReader#getImageCount() */ public int getImageCount() { FormatTools.assertId(getCurrentFile(), true, 2); return (reader.isRGB() && !reader.isIndexed()) ? reader.getRGBChannelCount() * reader.getImageCount() : reader.getImageCount(); } /* @see IFormatReader#getDimensionOrder() */ public String getDimensionOrder() { FormatTools.assertId(getCurrentFile(), true, 2); String order = super.getDimensionOrder(); if (reader.isRGB() && !reader.isIndexed()) { String newOrder = "XYC"; if (order.indexOf("Z") > order.indexOf("T")) newOrder += "TZ"; else newOrder += "ZT"; return newOrder; } return order; } /* @see IFormatReader#isRGB() */ public boolean isRGB() { FormatTools.assertId(getCurrentFile(), true, 2); return isIndexed() && !isFalseColor() && getSizeC() > 1; } /* @see IFormatReader#openBytes(int) */ public byte[] openBytes(int no) throws FormatException, IOException { return openBytes(no, 0, 0, getSizeX(), getSizeY()); } /* @see IFormatReader#openBytes(int, byte[]) */ public byte[] openBytes(int no, byte[] buf) throws FormatException, IOException { return openBytes(no, buf, 0, 0, getSizeX(), getSizeY()); } /* @see IFormatReader#openBytes(int, int, int, int, int) */ public byte[] openBytes(int no, int x, int y, int w, int h) throws FormatException, IOException { byte[] buf = new byte[w * h * FormatTools.getBytesPerPixel(getPixelType())]; return openBytes(no, buf, x, y, w, h); } /* @see IFormatReader#openBytes(int, byte[], int, int, int, int) */ public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.assertId(getCurrentFile(), true, 2); FormatTools.checkPlaneNumber(this, no); if (reader.isRGB() && !reader.isIndexed()) { int c = getSizeC() / reader.getEffectiveSizeC(); int source = getOriginalIndex(no); int channel = no % c; int series = getSeries(); int bpp = FormatTools.getBytesPerPixel(getPixelType()); if (source != lastImageIndex || series != lastImageSeries || x != lastImageX || y != lastImageY || w != lastImageWidth || h != lastImageHeight) { int strips = 1; // check how big the original image is; if it's larger than the // available memory, we will need to split it into strips Runtime rt = Runtime.getRuntime(); long availableMemory = rt.freeMemory(); if (availableMemory < w * h * bpp * c) { strips = (int) Math.sqrt(h); } int stripHeight = h / strips; int lastStripHeight = stripHeight + (h - (stripHeight * strips)); byte[] strip = strips == 1 ? buf : new byte[stripHeight * w * bpp]; for (int i=0; i<strips; i++) { lastImage = reader.openBytes(source, x, y + i * stripHeight, w, i == strips - 1 ? lastStripHeight : stripHeight); lastImageIndex = source; lastImageSeries = series; lastImageX = x; lastImageY = y + i * stripHeight; lastImageWidth = w; lastImageHeight = i == strips - 1 ? lastStripHeight : stripHeight; if (strips != 1 && lastStripHeight != stripHeight && i == strips - 1) { strip = new byte[lastStripHeight * w * bpp]; } ImageTools.splitChannels(lastImage, strip, channel, c, bpp, false, isInterleaved(), strips == 1 ? w * h * bpp : strip.length); if (strips != 1) { System.arraycopy(strip, 0, buf, i * stripHeight * w * bpp, strip.length); } } } else { ImageTools.splitChannels(lastImage, buf, channel, c, bpp, false, isInterleaved(), w * h * bpp); } return buf; } return reader.openBytes(no, buf, x, y, w, h); } /* @see loci.formats.IFormatReader#openThumbBytes(int) */ public byte[] openThumbBytes(int no) throws FormatException, IOException { FormatTools.assertId(getCurrentFile(), true, 2); int source = getOriginalIndex(no); byte[] thumb = reader.openThumbBytes(source); int c = getSizeC() / reader.getEffectiveSizeC(); int channel = no % c; int bpp = FormatTools.getBytesPerPixel(getPixelType()); return ImageTools.splitChannels(thumb, channel, c, bpp, false, false); } /* @see IFormatReader#close(boolean) */ public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { lastImage = null; lastImageIndex = -1; lastImageSeries = -1; lastImageX = -1; lastImageY = -1; lastImageWidth = -1; lastImageHeight = -1; } } public int getIndex(int z, int c, int t) { return FormatTools.getIndex(this, z, c, t); } public int[] getZCTCoords(int index) { return FormatTools.getZCTCoords(this, index); } // -- IFormatHandler API methods -- /* @see IFormatHandler#getNativeDataType() */ public Class<?> getNativeDataType() { return byte[].class; } /* @see IFormatHandler#setId(String) */ public void setId(String id) throws FormatException, IOException { super.setId(id); // clear last image cache lastImage = null; lastImageIndex = -1; lastImageSeries = -1; lastImageX = -1; lastImageY = -1; lastImageWidth = -1; lastImageHeight = -1; } }