// // AliconaReader.java // /* OME Bio-Formats package for reading and converting biological file formats. Copyright (C) 2005-@year@ UW-Madison LOCI and Glencoe Software, Inc. This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA */ package loci.formats.in; import java.io.IOException; import loci.common.RandomAccessInputStream; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; import ome.xml.model.primitives.PositiveFloat; /** * AliconaReader is the file format reader for Alicona AL3D files. * * <dl><dt><b>Source code:</b></dt> * <dd><a href="http://trac.openmicroscopy.org.uk/ome/browser/bioformats.git/components/bio-formats/src/loci/formats/in/AliconaReader.java">Trac</a>, * <a href="http://git.openmicroscopy.org/?p=bioformats.git;a=blob;f=components/bio-formats/src/loci/formats/in/AliconaReader.java;hb=HEAD">Gitweb</a></dd></dl> */ public class AliconaReader extends FormatReader { // -- Constants -- public static final String AL3D_MAGIC_STRING = "Alicona"; // -- Fields -- /** Image offset. */ private int textureOffset; /** Number of bytes per pixel (either 1 or 2). */ private int numBytes; // -- Constructor -- /** Constructs a new Alicona reader. */ public AliconaReader() { super("Alicona AL3D", "al3d"); domains = new String[] {FormatTools.SEM_DOMAIN}; } // -- IFormatReader API methods -- /* @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ public boolean isThisType(RandomAccessInputStream stream) throws IOException { final int blockLen = 16; if (!FormatTools.validStream(stream, blockLen, false)) return false; return (stream.readString(blockLen)).indexOf(AL3D_MAGIC_STRING) >= 0; } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); int pad = (8 - (getSizeX() % 8)) % 8; int planeSize = (getSizeX() + pad) * getSizeY(); if (getPixelType() == FormatTools.FLOAT) { in.seek(textureOffset); readPlane(in, x, y, w, h, buf); return buf; } // 16-bit images are stored in a non-standard format: // all of the LSBs are stored together, followed by all of the MSBs // so instead of LMLMLM... storage, we have LLLLL...MMMMM... for (int i=0; i<numBytes; i++) { in.seek(textureOffset + (no * planeSize * (i + 1))); in.skipBytes(y * (getSizeX() + pad)); if (getSizeX() == w) { in.read(buf, i * w * h, w * h); } else { for (int row=0; row<h; row++) { in.skipBytes(x); in.read(buf, i * w * h + row * w, w); in.skipBytes(getSizeX() + pad - x - w); } } } if (numBytes > 1) { byte[] tmp = new byte[buf.length]; for (int i=0; i<planeSize; i++) { for (int j=0; j<numBytes; j++) { tmp[i*numBytes + j] = buf[planeSize*j + i]; } } System.arraycopy(tmp, 0, buf, 0, tmp.length); tmp = null; } return buf; } /* @see loci.formats.IFormatReader#close(boolean) */ public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { textureOffset = numBytes = 0; } } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ protected void initFile(String id) throws FormatException, IOException { super.initFile(id); in = new RandomAccessInputStream(id); // check that this is a valid AL3D file LOGGER.info("Verifying Alicona format"); String magicString = in.readString(17); if (!magicString.trim().equals("AliconaImaging")) { throw new FormatException("Invalid magic string : " + "expected 'AliconaImaging', got " + magicString); } // now we read a series of tags // each one is 52 bytes - 20 byte key + 30 byte value + 2 byte CRLF LOGGER.info("Reading tags"); int count = 2; boolean hasC = false; String voltage = null, magnification = null, workingDistance = null; String pntX = null, pntY = null; int depthOffset = 0; for (int i=0; i<count; i++) { String key = in.readString(20).trim(); String value = in.readString(30).trim(); addGlobalMeta(key, value); in.skipBytes(2); if (key.equals("TagCount")) count += Integer.parseInt(value); else if (key.equals("Rows")) core[0].sizeY = Integer.parseInt(value); else if (key.equals("Cols")) core[0].sizeX = Integer.parseInt(value); else if (key.equals("NumberOfPlanes")) { core[0].imageCount = Integer.parseInt(value); } else if (key.equals("TextureImageOffset")) { textureOffset = Integer.parseInt(value); } else if (key.equals("TexturePtr") && !value.equals("7")) hasC = true; else if (key.equals("Voltage")) voltage = value; else if (key.equals("Magnification")) magnification = value; else if (key.equals("PixelSizeXMeter")) pntX = value; else if (key.equals("PixelSizeYMeter")) pntY = value; else if (key.equals("WorkingDistance")) workingDistance = value; else if (key.equals("DepthImageOffset")) { depthOffset = Integer.parseInt(value); } } LOGGER.info("Populating metadata"); if (textureOffset != 0) { numBytes = (int) (in.length() - textureOffset) / (getSizeX() * getSizeY() * getImageCount()); core[0].sizeC = hasC ? 3 : 1; core[0].sizeZ = 1; core[0].sizeT = getImageCount() / getSizeC(); core[0].pixelType = FormatTools.pixelTypeFromBytes(numBytes, false, false); } else { textureOffset = depthOffset; core[0].pixelType = FormatTools.FLOAT; core[0].sizeC = 1; core[0].sizeZ = 1; core[0].sizeT = 1; core[0].imageCount = 1; } core[0].rgb = false; core[0].interleaved = false; core[0].littleEndian = true; core[0].dimensionOrder = "XYCTZ"; core[0].metadataComplete = true; core[0].indexed = false; core[0].falseColor = false; MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this); // populate Image data MetadataTools.setDefaultCreationDate(store, id, 0); if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) { // link Image and Instrument String instrumentID = MetadataTools.createLSID("Instrument", 0); store.setInstrumentID(instrumentID, 0); store.setImageInstrumentRef(instrumentID, 0); // populate Detector data // According to the spec, the voltage and magnification values are those // used when the dataset was acquired, i.e. detector settings. if (voltage != null) { store.setDetectorSettingsVoltage(new Double(voltage), 0, 0); // link DetectorSettings to an actual Detector String detectorID = MetadataTools.createLSID("Detector", 0, 0); store.setDetectorID(detectorID, 0, 0); store.setDetectorSettingsID(detectorID, 0, 0); // set required Detector type store.setDetectorType(getDetectorType("Other"), 0, 0); } // populate Objective data if (magnification != null) { store.setObjectiveCalibratedMagnification( new Double(magnification), 0, 0); } if (workingDistance != null) { store.setObjectiveWorkingDistance(new Double(workingDistance), 0, 0); } store.setObjectiveCorrection(getCorrection("Other"), 0, 0); store.setObjectiveImmersion(getImmersion("Other"), 0, 0); // link Objective to an Image using ObjectiveSettings String objectiveID = MetadataTools.createLSID("Objective", 0, 0); store.setObjectiveID(objectiveID, 0, 0); store.setImageObjectiveSettingsID(objectiveID, 0); // populate Dimensions data if (pntX != null && pntY != null) { double pixelSizeX = Double.parseDouble(pntX) * 1000000; double pixelSizeY = Double.parseDouble(pntY) * 1000000; store.setPixelsPhysicalSizeX(new PositiveFloat(pixelSizeX), 0); store.setPixelsPhysicalSizeY(new PositiveFloat(pixelSizeY), 0); } } } }