// // OxfordInstrumentsReader.java // /* OME Bio-Formats package for reading and converting biological file formats. Copyright (C) 2005-@year@ UW-Madison LOCI and Glencoe Software, Inc. This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA */ package loci.formats.in; import java.io.IOException; import loci.common.RandomAccessInputStream; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; import ome.xml.model.primitives.PositiveFloat; /** * OxfordInstrumentsReader is the file format reader for * Oxford Instruments .top files. * * <dl><dt><b>Source code:</b></dt> * <dd><a href="http://trac.openmicroscopy.org.uk/ome/browser/bioformats.git/components/bio-formats/src/loci/formats/in/OxfordInstrumentsReader.java">Trac</a>, * <a href="http://git.openmicroscopy.org/?p=bioformats.git;a=blob;f=components/bio-formats/src/loci/formats/in/OxfordInstrumentsReader.java;hb=HEAD">Gitweb</a></dd></dl> */ public class OxfordInstrumentsReader extends FormatReader { // -- Constants -- public static final String OXFORD_MAGIC_STRING = "Oxford Instruments"; // -- Fields -- private long headerSize = 0; // -- Constructor -- /** Constructs a new Oxford Instruments reader. */ public OxfordInstrumentsReader() { super("Oxford Instruments", "top"); suffixNecessary = false; domains = new String[] {FormatTools.SPM_DOMAIN}; } // -- IFormatReader API methods -- /* @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ public boolean isThisType(RandomAccessInputStream stream) throws IOException { final int blockLen = OXFORD_MAGIC_STRING.length(); if (!FormatTools.validStream(stream, blockLen, false)) return false; return stream.readString(blockLen).equals(OXFORD_MAGIC_STRING); } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); in.seek(headerSize); readPlane(in, x, y, w, h, buf); return buf; } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ protected void initFile(String id) throws FormatException, IOException { super.initFile(id); in = new RandomAccessInputStream(id); core[0].littleEndian = true; in.order(isLittleEndian()); in.seek(48); String comment = in.readString(32); String dateTime = readDate(); in.skipBytes(8); double xSize = -in.readFloat() + in.readFloat(); in.skipBytes(20); double ySize = -in.readFloat() + in.readFloat(); in.skipBytes(24); double zMin = in.readFloat(); double zMax = in.readFloat(); in.skipBytes(864); core[0].sizeX = in.readInt(); core[0].sizeY = in.readInt(); in.skipBytes(28); if (getSizeX() == 0 && getSizeY() == 0) { core[0].sizeX = in.readInt(); core[0].sizeY = in.readInt(); in.skipBytes(196); } else in.skipBytes(204); core[0].pixelType = FormatTools.UINT16; core[0].sizeZ = 1; core[0].sizeC = 1; core[0].sizeT = 1; core[0].imageCount = 1; core[0].rgb = false; core[0].indexed = false; core[0].dimensionOrder = "XYZCT"; core[0].interleaved = false; if (FormatTools.getPlaneSize(this) + in.getFilePointer() > in.length()) { core[0].sizeY = 1; } int lutSize = in.readInt(); in.skipBytes(lutSize); headerSize = in.getFilePointer(); if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) { in.skipBytes(FormatTools.getPlaneSize(this)); int nMetadataStrings = in.readInt(); for (int i=0; i<nMetadataStrings; i++) { int length = in.readInt(); String s = in.readString(length); if (s.indexOf(":") != -1) { String key = s.substring(0, s.indexOf(":")).trim(); String value = s.substring(s.indexOf(":") + 1).trim(); if (!value.equals("-")) { addGlobalMeta(key, value); } } } addGlobalMeta("Description", comment); addGlobalMeta("Acquisition date", dateTime); addGlobalMeta("X size (um)", xSize); addGlobalMeta("Y size (um)", ySize); addGlobalMeta("Z minimum (um)", zMin); addGlobalMeta("Z maximum (um)", zMax); } MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this); store.setImageDescription(comment, 0); store.setImageAcquiredDate(dateTime, 0); double physicalSizeX = xSize / getSizeX(); double physicalSizeY = ySize / getSizeY(); if (physicalSizeX > 0) { store.setPixelsPhysicalSizeX(new PositiveFloat(physicalSizeX), 0); } if (physicalSizeY > 0) { store.setPixelsPhysicalSizeY(new PositiveFloat(physicalSizeY), 0); } } // -- Helper methods -- private String readDate() throws IOException { StringBuffer dateTime = new StringBuffer(); dateTime.append(String.valueOf(in.readInt())); // year dateTime.append("-"); int month = in.readInt(); dateTime.append(String.format("%02d", month)); dateTime.append("-"); int day = in.readInt(); dateTime.append(String.format("%02d", day)); dateTime.append("T"); int hour = in.readInt(); dateTime.append(String.format("%02d", hour)); dateTime.append(":"); int minute = in.readInt(); dateTime.append(String.format("%02d", minute)); dateTime.append(":"); in.skipBytes(4); float scanTime = in.readInt() / 100f; dateTime.append(String.format("%02d", (int) scanTime)); addGlobalMeta("Scan time (s)", scanTime); return dateTime.toString(); } }