// // FEIReader.java // /* OME Bio-Formats package for reading and converting biological file formats. Copyright (C) 2005-@year@ UW-Madison LOCI and Glencoe Software, Inc. This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA */ package loci.formats.in; import java.io.IOException; import loci.common.RandomAccessInputStream; import loci.formats.FormatException; import loci.formats.FormatReader; import loci.formats.FormatTools; import loci.formats.MetadataTools; import loci.formats.meta.MetadataStore; /** * FEIReader is the file format reader for FEI and Philips .img files. * * <dl><dt><b>Source code:</b></dt> * <dd><a href="http://trac.openmicroscopy.org.uk/ome/browser/bioformats.git/components/bio-formats/src/loci/formats/in/FEIReader.java">Trac</a>, * <a href="http://git.openmicroscopy.org/?p=bioformats.git;a=blob;f=components/bio-formats/src/loci/formats/in/FEIReader.java;hb=HEAD">Gitweb</a></dd></dl> */ public class FEIReader extends FormatReader { // -- Constants -- public static final String FEI_MAGIC_STRING = "XL"; private static final int INVALID_PIXELS = 112; // -- Fields -- private int headerSize; // -- Constructor -- /** Constructs a new FEI reader. */ public FEIReader() { super("FEI/Philips", "img"); suffixSufficient = false; domains = new String[] {FormatTools.SEM_DOMAIN}; } // -- IFormatReader API methods -- /* @see loci.formats.IFormatReader#isThisType(RandomAccessInputStream) */ public boolean isThisType(RandomAccessInputStream stream) throws IOException { final int blockLen = 2; if (!FormatTools.validStream(stream, blockLen, false)) return false; return stream.readString(blockLen).startsWith(FEI_MAGIC_STRING); } /** * @see loci.formats.IFormatReader#openBytes(int, byte[], int, int, int, int) */ public byte[] openBytes(int no, byte[] buf, int x, int y, int w, int h) throws FormatException, IOException { FormatTools.checkPlaneParameters(this, no, buf.length, x, y, w, h); in.seek(headerSize); byte[] segment = new byte[getSizeX() / 2]; byte[] plane = new byte[FormatTools.getPlaneSize(this)]; // interlace frames - there are four rows of two columns int halfRow = getSizeX() / 2; for (int q=0; q<4; q++) { for (int row=q; row<getSizeY(); row+=4) { for (int s=0; s<2; s++) { in.read(segment); in.skipBytes(INVALID_PIXELS / 2); for (int col=s; col<getSizeX(); col+=2) { plane[row*getSizeX() + col] = segment[col / 2]; } } } } RandomAccessInputStream pixels = new RandomAccessInputStream(plane); readPlane(pixels, x, y, w, h, buf); pixels.close(); return buf; } /* @see loci.formats.IFormatReader#close(boolean) */ public void close(boolean fileOnly) throws IOException { super.close(fileOnly); if (!fileOnly) { headerSize = 0; } } // -- Internal FormatReader API methods -- /* @see loci.formats.FormatReader#initFile(String) */ protected void initFile(String id) throws FormatException, IOException { super.initFile(id); in = new RandomAccessInputStream(id); in.order(true); LOGGER.info("Reading file header"); if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) { in.skipBytes(44); float magnification = in.readFloat(); float kV = in.readFloat() / 1000; float wd = in.readFloat(); in.skipBytes(12); float spot = in.readFloat(); addGlobalMeta("Magnification", magnification); addGlobalMeta("kV", kV); addGlobalMeta("Working distance", wd); addGlobalMeta("Spot", spot); } in.seek(514); core[0].sizeX = in.readShort() - INVALID_PIXELS; core[0].sizeY = in.readShort(); in.skipBytes(4); headerSize = in.readShort(); // always one grayscale plane per file core[0].sizeZ = 1; core[0].sizeC = 1; core[0].sizeT = 1; core[0].imageCount = 1; core[0].littleEndian = true; core[0].pixelType = FormatTools.UINT8; core[0].rgb = false; core[0].indexed = false; core[0].interleaved = false; core[0].dimensionOrder = "XYCZT"; MetadataStore store = makeFilterMetadata(); MetadataTools.populatePixels(store, this); MetadataTools.setDefaultCreationDate(store, id, 0); } }